The development of the IB Biology: Yeast and Catalase Experiment Following a teacher demonstration of measuring the rate of hydrogen peroxide and catalase enzyme students carry out a circus of three simple enzyme experiments.
Average final volumes of oxygen for each concentration of hydrogen peroxide. Evaluation Overall, I believe my experiment went well and that I gained Enzyme coursework ib results because I repeated each concentration three times and investigated eight concentrations in total.
I believe that my results were also relatively reliable because as the concentration decreased the volume of oxygen produced also decreased.
Also, most of the points were on or close to the curve of best fit for each concentration. However, there are some factors that I must take into consideration.
Apparatus Limitations Firstly, there were limitations on the apparatus that I used. Each piece of apparatus has an apparatus error with an upper and lower limit.
This obviously affects the amount of catalase present, which means that there could be more or fewer collisions and resulting successful collisions between enzyme and substrate molecules depending on the greater or lower mass of yeast.
For example, if there were more molecules of yeast, the rate of reaction would increase because there would be more collisions between enzyme and substrate molecules. This would result in a greater probability of successful collisions, and therefore more enzyme-substrate complexes being produced.
This means that in my results, the volume of gas produced in the first 5 seconds may have been higher than it should have been if I had used exactly 0. The same idea applies to the substrate concentration in that the pipettes also had an apparatus error.
This means the amount of substrate could have been different for each repeat, even though I used the same concentration. So in cm3, the actual volume could have been either If there were fewer molecules of hydrogen peroxide, there would have been fewer collisions between molecules of enzyme and substrate, resulting in fewer enzyme-substrate complexes being made.
However, I do not believe the substrate concentrations were significantly different because my repeats were mostly concordant, so a similar amount of oxygen was produced which must mean that there was a similar number of substrate molecules in each concentration.
Choice of Method I tried to select the method I considered would be most accurate. I decided on the gas syringe method because, as I explained in my section on preliminary work, it measured the volume of gas directly and minimised the volume of oxygen which could potentially dissolve in water.
However, some oxygen was displaced in the gas syringe and I had to solve this by subtracting this small amount from the volumes produced in each of the reactions. Also, I noticed if the barrel was wet, the syringe often got stuck for a short time before it recorded the volumes of gas.
To prevent this I had to dry out the barrel and syringe before commencing the procedure.
It was very hard to insert the small 5cm3 beaker into the conical flask, and when it came to tipping it over, some of the substrate was still trapped inside the beaker.
I solved this by swirling the conical flask constantly throughout the reactions, which seemed to solve the problem, although this meant that the amount of swirling had to be the same in order to ensure a fair test.Obtaining rate data, interpreting rate data, orders of reaction and rate expressions a.
Examples of obtaining rate data. A BRIEF REVIEW OF METHODS OF COLLECTING RATE DATA. The speed or rate of reaction is the rate of removal of reactant or the rate of formation of product. Biology Enzymes Coursework Prediction: I think that the enzyme will work best at c to c I think this because that optimum temperature for most natural enzymes is c but his is a chemical enzyme so it will work best a little higher.
Temperature and enzyme activity Aim: To determine the effect of which the temperature of the enzyme has on the rate of the enzyme catalysed reaction Hypothesis: The rate of reaction of an enzyme catalysed reaction will increase as the temperature of the enzyme approaches the optimum temperature.
Extracts from this document Introduction. The Effect of Concentration on Pectinase Using Apple Hypothesis: As the concentration of pectinase added to apple pulp increases, the faster the rate at which apple juice is produced.
Investing Rate of Reaction. I am going to investigate the rate of reaction between two substances: calcium carbonate and hydrochloric acid. Rate of reaction measures the . Port Manteaux churns out silly new words when you feed it an idea or two.
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